Chemical imaging of functional group distributions in living biofilms using infrared microspectroscopy
نویسندگان
چکیده
Bacteria predominantly grow as biofilms attached to liquid or solid interfaces. Biofilms are complex structures that contain bacterial cells interwoven in an extracellular polysaccharide matrix. The protein, lipid and polysaccharide molecules within a biofilm contain characteristic functional groups which can be identified and mapped using infrared microspectroscopy. The objective of this study is to image the distributions of biomolecules within living biofilms and to monitor the chemical evolution of biofilms in situ during microbial growth.
منابع مشابه
Molecular mapping of periodontal tissues using infrared microspectroscopy
BACKGROUND: Chronic periodontitis is an inflammatory disease of the supporting structures of the teeth. Infrared microspectroscopy has the potential to simultaneously monitor multiple disease markers, including cellular infiltration and collagen catabolism, and hence differentiate diseased and healthy tissues. Therefore, our aim was to establish an infrared microspectroscopy methodology with wh...
متن کاملSingle Cancer Cell Detection by Near Infrared Microspectroscopy, Infrared Chemical Imaging and Fluorescence Microspectroscopy
...............................................................................................1
متن کاملHighly resolved chemical imaging of living cells by using synchrotron infrared microspectrometry.
Using synchrotron radiation as an ultra-bright infrared source, we have been able to map the distributions of functional groups such as proteins, lipids, and nucleic acids inside a single living cell with a spatial resolution of a few microns. In particular, we have mapped the changes in the lipid and protein distributions in both the final stages of cell division and also during necrosis.
متن کاملNear Infrared Microspectroscopy, Fluorescence/Infrared Chemical Imaging and High-Resolution Nuclear Magnetic Resonance Analysi
...............................................................................................1
متن کاملImaging in living cells using νB-H Raman spectroscopy: monitoring COSAN uptake.
The boron-rich cobaltabisdicarbollide (COSAN) and its 8,8'-I2 derivative (I2-COSAN), both of purely inorganic nature, are shown to accumulate within living cells, where they can be detected using νB-H Raman microspectroscopy. This demonstrates an alternative method for cell labelling and detection.
متن کامل